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KMID : 0811720210250040297
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Korean Journal of Physiology & Pharmacology
2021 Volume.25 No. 4 p.297 ~ p.305
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Luteolin inhibits H2O2-induced cellular senescence via modulation of SIRT1 and p53
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Zhu Ri Zhe
Li Bing Si
Gao Shang Shang
Seo Jae-Ho
Choi Byung-Min
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Abstract
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Luteolin, a sort of flavonoid, has been reported to be involved in neuroprotective function via suppression of neuroinflammation. In this study, we investigated the protective effect of luteolin against oxidative stress-induced cellular senescence and its molecular mechanism using hydrogen peroxide (H2O2)-induced cellular senescence model in House Ear Institute-Organ of Corti 1 cells (HEI-OC1). Our results showed that luteolin attenuated senescent phenotypes including alterations of morphology, cell proliferation, senescence-associated β-galactosidase expression, DNA damage, as well as related molecules expression such as p53 and p21 in the oxidant challenged model. Interestingly, we found that luteolin induces expression of sirtuin 1 in dose- and time-dependent manners and it has protective role against H2O2-induced cellular senescence by upregulation of sirtuin 1 (SIRT1). In contrast, the inhibitory effect of luteolin on cellular senescence under oxidative stress was abolished by silencing of SIRT1. This study indicates that luteolin effectively protects against oxidative stress-induced cellular senescence through p53 and SIRT1. These results suggest that luteolin possesses therapeutic potentials against age-related hearing loss that are induced by oxidative stress.
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KEYWORD
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Cellular senescence, Hydrogen peroxide, Luteolin, Sirtuin 1, Tumor suppressor protein p53
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